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KMID : 1138520030060020077
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Journal of Pharmacopuncture
2003 Volume.6 No. 2 p.77 ~ p.90
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The Effect of Bee Venom on COX-2, P38, ERK and JNK in RAW 264.7 Cells
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Sim Jae-Young
Jo Hyun-Chol
Lee Seong-No
Kim Kee-Hyun
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Abstract
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The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide(H_2O_2)-induced expressions of cyclooxygenase-2(COX-2), p38, jun N-terminal Kinase(JNK) and extra-signal response kinase(ERK) in RAW 264.7 cells, a murine macrophage cell line.
Method : The expressions of COX-2, p38, JNK and ERK were determined by western blotting with corresponding antibodies.
Results : 1. The 0.5, 1 and 5;{mu}g/ml of bee venom inhibited significantly LPS and SNP-induced expression of COX-2 compared with control, respectively. The 0.5, 1 and 5;{mu}g/ml of bee venom inhibited insignificantly H_2O_2-induced expression of COX-2 compared with control, respectively. 2. The 0.5, 1 and 5;{mu}g/ml of bee venom inhibited significantly LPS, SNP and H_2O_2-induced expression of p38 compared with control, respectively. 3. The 1 and 5;{mu}g/ml of bee venom inhibited significantly SNP-induced expression of JNK compared with control, respectively. All of bee venom inhibited insignificantly LPS and H_2O_2-induced expression of JNK compared with control, respectively. 4. The 5;{mu}g/ml of bee venom inhibited significantly SNP-induced expression of ERK, the 0.5;{mu}g/ml of bee venom increased significantly H_2O_2-induced expression of ERK compared with control. The 0.5, 1 and 5;{mu}g/ml of bee venom inhibited insignificantly LPS-induced expression of ERK compared with control, respectively.
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KEYWORD
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Bee venom, COX-2, P38, ERK, JNK, RAW 264.7 cell, LPS, SNP, H_2O_2
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